Geographic Distribution and Evaluation of Saccharomyces cerevisiae Strains Isolated from Vineyards in the Warmer, Inland Regions of the Western Cape in South Africa

  • W. Khan Vine and Wine Research Institute, Nietvoorbij Centre for Vine and Wine, Stellenbosch
  • O.P.H. Augustyn ARC-Fruit, Vine and Wine Research Institute, Nietvoorbij Centre for Vine and Wine, Private Bag X5026, 7599 Stellenbosch, South Africa
  • T.J. van der Westhuizen Anchor Yeast, P.O. Box 14, 7475 Eppindust (Cape Town), South Africa
  • M.G. Lambrechts Institute for Wine Biotechnology, Department of Viticulture and Oenology, University of Stellenbosch, Private Bag XI, 7602 Matieland (Stellenbosch), South Africa
  • I.S. Pretorius Institute for Wine Biotechnology, Department of Viticulture and Oenology, University of Stellenbosch, Private Bag XI, 7602 Matieland (Stellenbosch), South Africa
DOI: https://doi.org/10.21548/21-1-2184

Abstract

The aim of this study was to examine the geographic distribution of Saccharomyces cerevisiae strains indigenous to 19 sites in the warmer, inland regions of the Western Cape in South Africa. These strains were compared to those isolated previously from the cooler, coastal regions of the same province by subjecting both sets of organisms to the same characterisation procedures. Thirty isolates per sampling site were isolated and the S. cerevisiae strains subjected to the following characterisation procedures; karyotyping using pulse field gel electrophoresis (CHEF), randomly amplified polymorphic DNA, the polymerase chain reaction technique (RAPD-PCR), sugar fermentation ability, flocculation ability, stress resistance/response and extracellular enzyme activity. When considering biodiversity per sampling site, CHEF karyotypes indicated the recovery of 30 S. cerevisiae strains. This number was reduced to 21 when comparing banding patterns over sites. Addition of RAPD-PCR data expanded the number of unique strains to 29. Subsequent consideration of sugar fermentation data indicated that one of the strians with exactly equivalent CHEF and RAPD-PCR patterns was in fact galactose positive while the other was galactose negative. These data clearly indicate that characterisation of yeast strains by application of a single technique is not a sound practice.  None of the S. cerevisiae strains isolated in this study occurred in the coastal regions. In addition, each site sampled in this study had its own unique collection of wine yeast strains and no strain common to all sites in the study region was found. Survival mechanisms of S. cerevisiae are obscure. Although we found that many of the isolated strains could grow invasively/form pseudohyphae and that these abilities could therefore contribute to the organism's overwintering ability, other mechanisms must also be involved.

Downloads

Download data is not yet available.
Published
2017-05-03
Issue
Vol. 21 No. 1 (2000)
Section
Articles

Copyright (c) 2017 South African Society for Enology and Viticulture

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

A copyright form will be e-mailed to the corresponding author when the manuscript has been accepted for publication.

 

In principle, the Author agrees to the following when he/she signes the copyright agreement:

I hereby assign to the SOUTH AFRICAN SOCIETY FOR ENOLOGY AND VITICULTURE (SASEV) the copyright of the text, tables, figures, supplementary material, illustrations and other information (the Material) submitted with the manuscript to be published in SOUTH AFRICAN JOURNAL OF ENOLOGY AND VITICULTURE (SAJEV) (the "Article"). The copyright becomes effective from the date the Article has been accepted for publication in SAJEV.

 

This is an open access journal, and the authors and journal should be properly acknowledged, when works are cited.

Author's may use the publishers version for teaching purposes, in books, theses, dissertations, conferences and conference papers.

A copy of the authors' publishers version may also be hosted on the following websites:

  • Non-commercial personal webpage or blog.
  • Institutional webpage.
  • Authors Institutional Repository.

The following notice should accompany such a posting on the website: This is an electronic version of an article published in SAJEV, Volume XXX, number XXX, pages XXX - XXX, DOI.   Authors should also supply a hyperlink to the original paper or indicate where the original paper (www.journals.ac.za/index.php/sajev/) may be found.

Authors publishers version, affiliated with the Stellenbosch University will be automatically deposited in the University's Institutional Repository SUNScholar.

Articles as a whole, may not be re-published with another journal.

The following license applies:

Attribution CC BY-NC-ND 4.0