Fingerprinting and Identification of Bacteria Present in UASB Granules Used to Treat Winery, Brewery, Distillery or Peach-lye Canning Wastewater

  • M. Keyser Department of Food Science, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa
  • T.J. Britz Department of Food Science, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa
  • R.C. Witthuhn Department of Food Science, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa
DOI: https://doi.org/10.21548/28-1-1462

Abstract

The effective operation of the anaerobic digestion process in an upflow anaerobic sludge blanket (UASB) bioreactor
is dependent on the microbial composition of the UASB granules. The granules contain a consortium of bacteria,
with a specific metabolic function for each group, contributing to the overall efficiency and stability of the
bioreactor. The aim of this study was to fingerprint and identify the bacteria present in four different types of South
African UASB granules that are used to treat winery, brewery, distillery and peach-lye canning wastewaters. This
was done by combining conventional microbiological platings with PCR-based denaturing gradient gel
electrophoresis (DGGE) and DNA sequence analysis. Each granule type showed distinct PCR-based DGGE
fingerprints with unique bands, while other bands were found to be present in all the granules, regardless of the
wastewater being treated. Sixty-eight different bacteria (40 pure isolates and 28 clones) were partially sequenced
and identified from the winery, brewery, distillery and peach-lye canning granules. Thirty-five percent of the
identified bacteria represented the unculturable bacteria and 65% represented the culturable bacteria, which
included members of the following genera: Bacillus, Pseudomonas, Bacteroides, Enterococcus, Alcaligenes,
Clostridium, Shewanella, Microbacterium, Leuconostoc, Sulfurospirillum, Acidaminococcus, Vibrio, Aeromonas,
Nitrospira, Synergistes, Rhodococcus, Rhodocyclus and Syntrophobacter. A DGGE marker was successfully
constructed, representing members of the bacterial consortium in UASB granules.

Downloads

Download data is not yet available.
Published
2016-12-13
Issue
Vol. 28 No. 1 (2007)
Section
Articles

A copyright form will be e-mailed to the corresponding author when the manuscript has been accepted for publication.

 

In principle, the Author agrees to the following when he/she signes the copyright agreement:

I hereby assign to the SOUTH AFRICAN SOCIETY FOR ENOLOGY AND VITICULTURE (SASEV) the copyright of the text, tables, figures, supplementary material, illustrations and other information (the Material) submitted with the manuscript to be published in SOUTH AFRICAN JOURNAL OF ENOLOGY AND VITICULTURE (SAJEV) (the "Article"). The copyright becomes effective from the date the Article has been accepted for publication in SAJEV.

 

This is an open access journal, and the authors and journal should be properly acknowledged, when works are cited.

Author's may use the publishers version for teaching purposes, in books, theses, dissertations, conferences and conference papers.

A copy of the authors' publishers version may also be hosted on the following websites:

  • Non-commercial personal webpage or blog.
  • Institutional webpage.
  • Authors Institutional Repository.

The following notice should accompany such a posting on the website: This is an electronic version of an article published in SAJEV, Volume XXX, number XXX, pages XXX - XXX, DOI.   Authors should also supply a hyperlink to the original paper or indicate where the original paper (www.journals.ac.za/index.php/sajev/) may be found.

Authors publishers version, affiliated with the Stellenbosch University will be automatically deposited in the University's Institutional Repository SUNScholar.

Articles as a whole, may not be re-published with another journal.

The following license applies:

Attribution CC BY-NC-ND 4.0