Evaluating the Efficacy of Lysozyme Against Lactic Acid Bacteria Under Different Winemaking Scenarios

  • M. Azzolini Centro per la Sperimentazione in Vitivinicoltura, Provincia di Verona, Servizio Agricoltura, Via della Pieve 64-37020 San Pietro in Cariano – Verona, Italy
  • E. Tosi Centro per la Sperimentazione in Vitivinicoltura, Provincia di Verona, Servizio Agricoltura, Via della Pieve 64-37020 San Pietro in Cariano – Verona, Italy
  • G. Veneri Dipartimento di Biotecnologie, Università degli Studi di Verona, strada Le Grazie 15, 37134 Verona, Italy
  • G. Zapparoli Dipartimento di Biotecnologie, Università degli Studi di Verona, strada Le Grazie 15, 37134 Verona, Italy

Abstract

This study evaluated the efficacy of lysozyme in winemaking to control lactic acid bacteria (LAB). In a winery
vinification, indigenous LAB were partially and completely inhibited when lysozyme was added to red and white
grape must respectively. This result was confirmed by using two selected strains of Lactobacillus brevis and
Oenococcus oeni to contaminate the grape must. In the red wine microvinification, the cell population decreased
only temporarily and malolactic fermentation terminated at different times, depending on the grape must pH and
lysozyme dosage. In the white wine microvinification, cell mortality rates differed according to lysozyme dosage
rather than pH values. During the fermentation, lysozyme activity was stable or decreased, depending on the
absence or presence of grape must respectively. The study highlighted that lysozyme efficacy is strongly affected by
the type of vinification.
Published
2016-12-12
Section
Articles