Screening Non-Saccharomyces Yeasts as Low Ethanol Producing Starter Cultures


Non-Saccharomyces yeasts are known for their low fermentation rate in comparison to Saccharomyces cerevisiae.  In this study, non-Saccharomyces yeasts were inoculated into Chenin blanc grape must and fermented under aerobic and anaerobic conditions.  Saccharomycodes ludwigii displayed a strain-dependent fermentation rate, which yielded between 5.2% and 9.9% ethanol concentration under both conditions, albeit with residual sugar.  Aerobic conditions favoured the production of reduced ethanol which was between 5.8% and 9.7% for non-Saccharomyces yeasts in comparison to S. cerevisiae (10%).  This trend was observed for Wickerhamomyces anomalus, Stamerella bacillaris (Candida zemplinina), Metshnikowia pulcherrima, Cyberlindnera saturnus, Wickerhamomyces subpelliculosus and Cyberlindnera jadinii.  The laboratory-scale wines prepared with the aforementioned yeasts yielded ripe fruit and floral aroma attributes while other non-Saccharomyces yeasts resulted in wines with spicy, acidic and solvent aroma notes.

Author Biographies

N.N. Mehlomakulu
Post-harvest and Agro-processing Technologies Division, ARC Infruitec-Nietvoorbij (The Fruit, Vine and Wine Institute of the Agricultural Research Council), Stellenbosch
J.W. Hoff
Post-harvest and Agro-processing Technologies Division, ARC Infruitec-Nietvoorbij (The Fruit, Vine and Wine Institute of the Agricultural Research Council), Stellenbosch
H. Erten
Department of Food Engineering, Faculty of Agriculture, Cukurova University
L. Beukes
Post-harvest and Agro-processing Technologies Division, ARC Infruitec-Nietvoorbij (The Fruit, Vine and Wine Institute of the Agricultural Research Council), Stellenbosch
N.P. Jolly, Post-harvest and Agro-processing TechnologiesARC Infruitec-NietvoorbijStellenboschSouth Africa
Senor Researcher (Microbiology), Post-harvest and Agro-processing Technologies, ARC Infruitec-Nietvoorbij, Stellenbosch


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