Anatomical responses to cytokinins of abscised grapevine shoot apices cultured in vitro
Abstract
Responses of excised shoot apices of V. vinifera L. cv. Chenin blanc to different cytokinin treatments were studied at various intervals (days) after the start of in vitro culture, using light and scanning electron microscopy. Results clearly indicated that in vitro produced shoots were of axillary origin. Shoot clusters were produced by the enhanced release of axillary meristems from apical dominance, due to the application of 6-benzylaminopurine (BAP) singly as well as in combination with zeatin riboside (ZR). Axillary meristems on these axillary shoots were subsequently released from apical dominance, thus giving rise to shoot clusters of high densities. Shoot clusters induced by ZR alone were less dense, probably due to an initial delay in elongation of axillary meristems nearest to the main apical meristem of the shoot. However, elongation of axillary meristems lower down the axes proceeded strongly in the presence of ZR. Application of BAP resulted in more pronounced release of axillary meristems from apical dominance than with ZR.Downloads
Copyright (c) 2017 South African Society for Enology and Viticulture

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
A copyright form will be e-mailed to the corresponding author when the manuscript has been accepted for publication.
In principle, the Author agrees to the following when he/she signes the copyright agreement:
I hereby assign to the SOUTH AFRICAN SOCIETY FOR ENOLOGY AND VITICULTURE (SASEV) the copyright of the text, tables, figures, supplementary material, illustrations and other information (the Material) submitted with the manuscript to be published in SOUTH AFRICAN JOURNAL OF ENOLOGY AND VITICULTURE (SAJEV) (the "Article"). The copyright becomes effective from the date the Article has been accepted for publication in SAJEV.
This is an open access journal, and the authors and journal should be properly acknowledged, when works are cited.
Author's may use the publishers version for teaching purposes, in books, theses, dissertations, conferences and conference papers.
A copy of the authors' publishers version may also be hosted on the following websites:
- Non-commercial personal webpage or blog.
- Institutional webpage.
- Authors Institutional Repository.
The following notice should accompany such a posting on the website: This is an electronic version of an article published in SAJEV, Volume XXX, number XXX, pages XXX - XXX, DOI. Authors should also supply a hyperlink to the original paper or indicate where the original paper (www.journals.ac.za/index.php/sajev/) may be found.
Authors publishers version, affiliated with the Stellenbosch University will be automatically deposited in the University's Institutional Repository SUNScholar.
Articles as a whole, may not be re-published with another journal.
The following license applies:
Attribution CC BY-NC-ND 4.0