ICP-MS Measurement of 11B/10B Isotope Ratios in Grapevine Leaves and the Investigation of Possible Boron Isotope Fractionation in Grapevine Plants
Abstract
The correlation between the 11B/10B ratio in grapevine leaves and that in the growth medium was establishedin a series of hydroponic experiments with grapevine cuttings for different cultivar/rootstocks combinations.
The hydroponic growth medium was alternately spiked with boric acid containing B with natural isotope
composition and B enriched in 10B, so as to vary the 11B/10B ratio. B isotope ratios in grapevine leaves were
determined by quadrupole-based ICP-MS after digestion and complete matrix removal through microwave
digestion and isolation of matrix-free B species using ion exchange separation. It was found that the B isotope
ratios in the leaves were not identical to those in the growth medium, but that a change in the ratio in the growth
medium induced a similar change in the leaves. For a particular cultivar/rootstock combination, a characteristic
B isotope ratio was found that was different from the ratio in a group of plants with a different cultivar/rootstock
combination.
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