Effect of Oxygen and Nitrogen Sparging during Grape Fermentation on Volatile Sulphur Compounds
AbstractElemental sulphur is a common fungicide applied in vineyards before harvest, and has been found toincrease the production of desirable polyfunctional mercaptans, but also H2S and unwanted reductivesulphur aroma compounds. This paper investigates the effectiveness of oxygen and nitrogen sparging,applied during fermentation, on the removal of volatile sulphur compounds in Sauvignon blanc wines.Increasing the amount of elemental sulphur added to grapes after pressing, from nil to 10 to 100 mg/L,led to an increase in the formation of 3-mercaptohexanol (3MH), of 3-mercaptohexyl acetate (3MHA) forthe 10 mg/L additions only, and of some unwanted reductive compounds. Few changes were observed inthe concentrations of aroma compounds when the juices were sparged with nitrogen during fermentation.Additions of oxygen during fermentation led to some decrease in the concentration of polyfunctionalmercaptans for the 10 mg/L sulphur additions, but did not significantly remove reductive aroma compounds.Few differences were observed in the concentration of wine phenolics or of further wine aroma familieswith any of the treatments.
Araujo, L. D., Vannevel, S., Buica, A., Callerot, S., Fedrizzi, B., Kilmartin, P. A., & du Toit, W. J., 2017. Indications of the prominent role of elemental sulfur in the formation of the varietal thiol 3-mercaptohexanol in Sauvignon blanc wine. Food Res. Int. 98, 79-86.
Bekker, M., Day, M., Holt, H., Wilkes, E., & Smith, P. A., 2016. Effect of oxygen exposure during fermentation on volatile sulfur compounds in S hiraz wine and a comparison of strategies for remediation of reductive character. Aust. J. Grape Wine Res. 22(1), 24-35.
Bekker, M., Espinase Nandorfy, D., Kulcsar, A., Faucon, A., Bindon, K., & Smith, P., 2021. Comparison of remediation strategies for decreasing ‘reductive’characters in Shiraz wines. Aust. J. Grape Wine Res. 27(1), 52-65.
Benkwitz, F., Nicolau, L., Lund, C., Beresford, M., Wohlers, M., & Kilmartin, P. A., 2012. Evaluation of key odorants in sauvignon blanc wines using three different methodologies. J. Agric. Food Chem. 60(25), 6293-6302.
Bertrand, A., & Torres-Alegre, V., 1984. Incidence de l'action de l'oxygène sur la formation des produits secondaires de la fermentation alcoolique du moût de raisin. Sci Aliments. 4(1), 45-64.
Burdock, G. (2005). Fenaroli's Handbook of Flavor Ingredients. In: Boca Raton, FL: CRC Press.
Chatonnet, P., Dubourdie, D., Boidron, J. n., & Pons, M., 1992. The origin of ethylphenols in wines. J. Sci. Food Agric. 60(2), 165-178.
Day, M., Schmidt, S., Smith, P., & Wilkes, E., 2015. Use and impact of oxygen during winemaking. Aust. J. Grape Wine Res. 21, 693-704.
Du Toit, W., Marais, J., Pretorius, I., & Du Toit, M., 2006. Oxygen in must and wine: A review. S. Afr. J. Enol. Vitic. 27(1), 76-94.
Fang, Y., & Qian, M. C., 2005. Sensitive quantification of sulfur compounds in wine by headspace solid-phase microextraction technique. J. Chromatogr. A 1080(2), 177-185.
Ferreira, B., Hory, C., Bard, M., Taisant, C., Olsson, A., & Le Fur, Y., 1995. Effects of skin contact and settling on the level of the C18: 2, C18: 3 fatty acids and C6 compounds in Burgundy Chardonnay musts and wines. Food Qual. Prefer. 6(1), 35-41.
Franco-Luesma, E., & Ferreira, V., 2016. Reductive off-odors in wines: Formation and release of H2S and methanethiol during the accelerated anoxic storage of wines. Food Chem. 199, 42-50.
García, E., Chacón, J., Martínez, J., & Izquierdo, P., 2003. Changes in volatile compounds during ripening in grapes of Airén, Macabeo and Chardonnay white varieties grown in La Mancha region (Spain). Food Sci. Technol. Int. 9(1), 33-41.
Gómez-Plaza, E., & Cano-López, M., 2011. A review on micro-oxygenation of red wines: Claims, benefits and the underlying chemistry. Food Chem. 125(4), 1131-1140.
Hebditch, K. R., Nicolau, L., & Brimble, M. A., 2007. Synthesis of isotopically labelled thiol volatiles and cysteine conjugates for quantification of Sauvignon Blanc wine. J. Label. Compd. Radiopharm. 50(4), 237-243.
Herbst-Johnstone, M., Araujo, L., Allen, T., Logan, G., Nicolau, L., & Kilmartin, P., 2012. Effects of mechanical harvesting on Sauvignon blanc aroma. International Workshop on Vineyard Mechanization and Grape and Wine Quality 978.
Herbst-Johnstone, M., Piano, F., Duhamel, N., Barker, D., & Fedrizzi, B., 2013. Ethyl propiolate derivatisation for the analysis of varietal thiols in wine. J. Chromatogr. A 1312, 104-110.
Iyer, M. M., Sacks, G. L., & Padilla-Zakour, O. I., 2010. Impact of harvesting and processing conditions on green leaf volatile development and phenolics in Concord grape juice. J. Food Sci. 75(3), C297-304.
Jastrzembski, J. A., Allison, R. B., Friedberg, E., & Sacks, G. L., 2017. Role of elemental sulfur in forming latent precursors of H2S in wine. J. Agric. Food Chem. 65(48), 10542-10549.
Jiang, B., & Zhang, Z., 2010. Volatile compounds of young wines from cabernet sauvignon, cabernet gernischet and chardonnay varieties grown in the loess plateau region of china. Molecules, 15(12), 9184-9196.
Jouanneau, S., Weaver, R. J., Nicolau, L., Herbst-Johnstone, M., Benkwitz, F., & Kilmartin, P. A., 2012. Subregional survey of aroma compounds in Marlborough Sauvignon Blanc wines. Aust. J. Grape Wine Res. 18(3), 329-343.
König, H., Unden, G., & Fröhlich, J., 2009. Biology of Microorganisms on Grapes, in Must and in Wine. Springer-Verlag, Heidelberg.
Kreitman, G. Y., Danilewicz, J. C., Jeffery, D. W., & Elias, R. J., 2016. Reaction mechanisms of metals with hydrogen sulfide and thiols in model wine. Part 1: Copper-catalyzed oxidation. J. Agric. Food Chem. 64(20), 4095-4104.
Kwasniewski, M. T., Sacks, G. L., & Wilcox, W. F., 2014. Persistence of elemental sulfur spray residue on grapes during ripening and vinification. Am. J. Enol. Vitic. 65(4), 453-462.
Lacroux, F., Trégoat, O., van Leeuwen, C., Pons, A., Tominaga, T., Lavigne-Cruège, V., & Dubourdieu, D., 2008. Effect of foliar nitrogen and sulphur application on aromatic expression of Vitis vinifera L. cv. Sauvignon blanc. OENO One, 42(3), 125-132.
Landaud, S., Helinck, S., & Bonnarme, P., 2008. Formation of volatile sulfur compounds and metabolism of methionine and other sulfur compounds in fermented food. Appl. Microbiol. Biotechnol. 77(6), 1191-1205.
Lytra, G., Tempere, S., de Revel, G., & Barbe, J.-C., 2012. Distribution and organoleptic impact of ethyl 2-hydroxy-4-methylpentanoate enantiomers in wine. J. Agric. Food Chem. 60(6), 1503-1509.
Lyu, X., Dias Araujo, L., Quek, S.-Y., & Kilmartin, P. A., 2021. Effects of antioxidant and elemental sulfur additions at crushing on aroma profiles of Pinot Gris, Chardonnay and Sauvignon Blanc Wines. Food Chem. 346, 128914.
Maggu, M., Winz, R., Kilmartin, P. A., Trought, M. C., & Nicolau, L., 2007. Effect of skin contact and pressure on the composition of Sauvignon Blanc must. J. Agric. Food Chem. 55(25), 10281-10288.
McCord, J., 2003. Application of toasted oak and micro-oxygenation to ageing of Cabernet Sauvignon wines. Aust. N.Z. Grapegrow. Winemak. 474, 43-53.
Mestres, M., Busto, O., & Guasch, J., 2000. Analysis of organic sulfur compounds in wine aroma. J. Chromatogr. A 881(1-2), 569-581.
Mestres, M., Busto, O., & Guasch, J., 2002. Application of headspace solid-phase microextraction to the determination of sulphur compounds with low volatility in wines. J. Chromatogr. A 945(1-2), 211-219.
Nguyen, D.-D., Nicolau, L., & Kilmartin, P. A., 2012. Application of an automated headspace solid phase micro-extraction for the GC-MS detection and quantification of reductive sulfur compounds in wines. In B. Salih (ed.), Gas chromatography in plant science, wine technology, toxicology and some specific applications pp. 171-195. InTech.
Nikolantonaki, M., Magiatis, P., & Waterhouse, A. L., 2014. Measuring protection of aromatic wine thiols from oxidation by competitive reactions vs wine preservatives with ortho-quinones. Food Chem. 163, 61-67.
Perestrelo, R., Fernandes, A., Albuquerque, F. F., Marques, J. C., & Câmara, J. S., 2006. Analytical characterization of the aroma of Tinta Negra Mole red wine: Identification of the main odorants compounds. Anal. Chim. Acta 563(1-2), 154-164.
Ribéreau-Gayon, P., Glories, Y., Maujean, A., & Dubourdieu, D., 2006 (2nd ed). Handbook of enology: The chemistry of wine stabilization and treatments, vol. 2. John Wiley & Sons Ltd, Chichester, UK.
Roland, A., Schneider, R., Razungles, A., & Cavelier, F., 2011. Varietal thiols in wine: discovery, analysis and applications. Chem. Rev. 111(11), 7355-7376.
Salmon, J.-M., 2006. Interactions between yeast, oxygen and polyphenols during alcoholic fermentations: Practical implications. LWT 39(9), 959-965.
Savocchia, S., Mandel, R., Crisp, P., & Scott, E., 2011. Evaluation of ‘alternative’materials to sulfur and synthetic fungicides for control of grapevine powdery mildew in a warm climate region of Australia. Australas. Plant Pathol. 40(1), 20-27.
Schneider, R., Charrier, F., Razungles, A., & Baumes, R., 2006. Evidence for an alternative biogenetic pathway leading to 3-mercaptohexanol and 4-mercapto-4-methylpentan-2-one in wines. Anal. Chim. Acta 563(1-2), 58-64.
Siebert, T. E., Solomon, M. R., Pollnitz, A. P., & Jeffery, D. W., 2010. Selective determination of volatile sulfur compounds in wine by gas chromatography with sulfur chemiluminescence detection. J. Agric. Food Chem. 58(17), 9454-9462.
Spedding, D., & Raut, P., 1982. The influence of dimethyl sulphide and carbon disulphide in the bouquet of wines. Vitis 21, 240-246.
Tandon, K., Baldwin, E., & Shewfelt, R., 2000. Aroma perception of individual volatile compounds in fresh tomatoes (Lycopersicon esculentum, Mill.) as affected by the medium of evaluation. Postharvest Biol. Technol. 20(3), 261-268.
Zhao, C. Y., Xue, J., Cai, X. D., Guo, J., Li, B., & Wu, S., 2016. Assessment of the key aroma compounds in rose-based products. J. Food Drug Anal. 24(3), 471-476.
Zoecklein, B., 2007. Factors impacting sulfur-like off odors in wine and winery options. 8th Annual Enology and Viticulture British Columbia Wine Grape Council Conference, Penticton, BC, Canada.
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