Hydric behaviour and gas exchange in different grapevine varieties (Vitis vinifera L.) from the Maule Valley (Chile)

  • G. Gutiérrez-Gamboa Universidad de Talca, Facultad de Ciencias Agrarias, Centro Tecnológico de la Vid y del Vino (CTVV)
  • A.G. Pérez-Donoso Pontificia Universidad Católica de Chile, Departamento de Fruticultura y Enología
  • A. Pou-Mir Instituto de Ciencias de la Vid y del Vino (CSIC, Gobierno de La Rioja, Universidad de La Rioja)
  • C. Acevedo-Opazo Universidad de Talca, Facultad de Ciencias Agrarias, CITRA
  • H. Valdés-Gómez Pontificia Universidad Católica de Chile, Departamento de Fruticultura y Enología


In the near future, stomatal behaviour will be crucial to counteract conditions arising from climate change.  Grapevine varieties are classified as either isohydric or anisohydric, depending on the sensitivity of stomata
to water deficit and on their water potential homeostasis. However, the great variability observed in different studies indicates that a continuum exists in the range of stomatal sensitivity to water stress. Thus, more knowledge about the hydric behaviour and the gas exchange of isohydric and anisohydric grapevine varieties under different water conditions could lead to the development of irrigation strategies oriented at improving water-use efficiency, yield and berry composition. In this study, research was conducted in order to characterise the stomatal regulation of four different Vitis vinifera L. varieties, namely Pinot noir, Sauvignon blanc, Chardonnay and Merlot, according to soil water status. Measurements of leaf gas exchange, together with measurements of stem water potential (Ψs) and leaf water potential (Ψl), were taken during two seasons. Under conditions of water stress, Chardonnay and Merlot reached a minimum Ψs of -1.67 and -1.68 MPa respectively, and higher levels of water-use efficiency (AN/gs), of 62.3 and 69.7 μmol CO2/mol H2O respectively. In Sauvignon blanc and Pinot noir, the minimum Ψs was -1.26 and -1.40 MPa
respectively, with lower levels of AN/gs (53.1 and 50.5 μmol CO2/mol H2O, respectively). Under conditions of water stress (Ψl < -0.9 MPa and Ψs < -0.6 MPa), all varieties had a significantly increased AN/gs1, despite
a significant reduction in gas exchange. Therefore, the hydric behaviour and gas exchange observed in this study suggest that Chardonnay and Merlot could be characterised as anisohydric varieties, as they present less sensitive stomatal control, while Pinot noir can be classified as a near-anisohydric variety and Sauvignon blanc as an isohydric variety. New investigations should consider other characteristics of the varieties to classify them better.


Acevedo-Opazo, C., Valdés-Gómez, H., Taylor, J.A., Avalo, A., Verdugo-Vásquez, N., Araya, M., Jara-Rojas, F.,  Tisseyre, B., 2013. Assessment of an empirical spatial prediction model of vine water status for irrigation management in a grapevine field. Agric. Water Manag. 124, 58-68.

Cifre, J., Bota, J., Escalona, J., Medrano, H., & Flexas, J., 2005. Physiological tools for irrigation scheduling in grapevine (Vitis vinifera L.). An open gate to improve water-use efficiency? Agric. Ecosyst. Environ. 106, 159-170.

Domec, J.C., & Johnson, D.M., 2012. Does homeostasis or disturbance of homeostasis in minimum leaf water potential explain the isohydric versus anisohydric behavior of Vitis vinifera L. cultivars? Tree Physiol. 32, 245-248.

Gómez del Campo, M., Ruiz, C., & Lissaguirre, J.R., 2002. Effect of water stress on leaf área development, photosynthesis and productivity in Chardonnay and Airen grapevines. Am. J. Enol. Vitic. 53, 138-143.

Jara-Rojas, F., Ortega-Farías, S., Valdéz-Gómez, H.,  Acevedo-Opazo, C., 2015. Gas exchange relations of ungrafted grapevines (cv. Carménère) growing under irrigated field conditions. S. Afric. J. Enol. Vitic. 36, 231-242.

Jones, H., 1998. Stomatal control of photosynthesis and transpiration. J. Exp. Bot. 49, 387-398.

Koundouras, S., van Leeuwen, C., Seguin, G.,  Gloires, Y., 1999. Influence de l'alimentation en eau sur la croissance de la vigne, la maturation des raisins et les caractéristiques des vins en zone méditerranéenne (Exemple de Némée, Grèce, cépage Saint Georges, 1997). J. Int. Sci. Vigne Vin. 33, 149-160.

Lebon, E., Pellegrino, A., Louarn, G.,  Lecoeur, J., 2006. Branch development controls leaf area dynamics in grapevine (Vitis vinifera) growing in drying soil. Ann. Bot. 98, 175-185.

Mccarthy, M., 2000. Developmental variation in sensitivity of Vitis vinifera L. (Shiraz) berries to soil water deficit. Aust. J. Grape Wine Res. 6, 136-140.

Medrano, H., Escalona, J., Bota, J., Gulias, J. & Flexas, J., 2002. Regulation of photosynthesis of C-3 plants in response to progressive drought: Stomatal conductance as a reference parameter. Ann. Bot. 89(7), 895-905.

Ojeda, H., Deloire, A., & Carbonneau, A., 2001. Influence of water stress deficits on grape berry growth. Vitis. 40, 141-145.

Ojeda, H., Andary, C., Kraeva, E., Carbonneau, A., & Deloire, A., 2002. Influence of pre-and postveraison water deficit on synthesis and concentration of skin phenolic compounds during berry growth of Vitis vinifera cv. Shiraz. Am. J. Enol. Vitic. 53, 261-267.

Ortega-Farías, S., Cuenca, R., Solís, B.,  Ortiz, C., 1997. Evaluación de la evapotranspiración de referencia usando la ecuación de Penman-Monteith. Cienc. Investig. Agrar. 23, 61-66.

Popescu, A., Enache, V., Simion, C., Donici, A.,  Tabaranu, G., 2009. Research concerning the economic impact of climate change upon grape production. Bulletin UASVM. 66, 366-372.

Pou, A., Medrano, I., Tomàs, M., Martorell, S., Ribas-Carbó, M.,  Flexas, J., 2012. Anisohydric behaviour in grapevines results in better performance under moderate water stress and recovery than isohydric behaviour. Plant Soil. 359, 335-349.

Prieto, J.A., Lebon, E.,  Ojeda, H., 2010. Stomatal behavior of different grapevine cultivars in response to soil water status and air water vapor pressure deficit. J. Int. Sci. Vigne Vin. 44, 9-20.

Sibille, I., Ojeda, H., Prieto, J., Maldonado, S., Lacapere, J. & Carbonneau, A., 2007. Relation between the values of three pressure chamber modalities (midday leaf, midday stem and predawn water potential) of 4 grapevine cultivars in drought situation of the southern France. Applications for irrigation control. Paper presented at the XVth International Symposium GESCO, June 2007, Porec, Croatia.

Soar, C.J., Speirs, J., Maffel, S.M., Penrose, A.B., Mccarthy, M.G., & Loveys, B.R., 2006. Grape vine varieties Shiraz and Grenache differ in their stomatal response to VPD: apparent links with ABA physiology and gene expression in leaf tissue. Aust. J. Grape Wine Res. 12, 2-12.

Tardieu, F., & Simonneau, T., 1998. Variability among species of stomatal control under fluctuating soil water status and evaporative demand: Modelling isohydric and anisohydric behaviours. J. Exp. Bot. 49, 419-432.

van Leeuwen, C., Tregoat, O., Choné, X., Bois, B., Pernet, D.,  Gaudillère, J.P., 2009. Vine water status is a key factor in grape ripening and vintage quality for red Bordeaux wine. How can it be assessed for vineyard management purposes? J. Int. Sci. Vigne Vin. 43, 121-134.

van Leeuwen, C.,  Darriet, P., 2016. The impact of climate change on viticulture and wine quality. Journal of Wine Economic. 11, 150-167.

West, A.G., Dawson, T.E., February, E.C., Midgley, G.F., Bond, W.J.,  Aston, T.L., 2012. Diverse functional responses to drought in a Mediterranean-type shrubland in South Africa. New Phytol. 195, 396-407.

Zufferey, W., Spring, J.L., Verdenal, T., Dienes, A., Belcher, S., Lorenzini, F., Koestel, C., Rösti, J., Gindro, J., Spangenberg, J.,  Viret, O., 2017. The influence of water stress on plant hydraulics, gas exchange, berry composition and quality of Pinot Noir wines in Switzerland. OENO One. 51, 17-27.