Analysis of SSR and AFLP Markers to Detect Genetic Diversity Among Selected Clones of Grapevine (Vitis vinifera L.) cv. Keshmeshi

  • H. Doulati Baneh Department of Seed and Plant Breeding, Agricultural and Natural Resource Research Centre of West Azerbaijan, P.O. Box 365, Uremia, Iran
  • S.A. Mohammadi Department of Agronomy & Plant Breeding, Faculty of Agriculture, University of Tabriz, Tabriz 51664, Iran
  • H. Mahmoudzadeh Department of Seed and Plant Breeding, Agricultural and Natural Resource Research Centre of West Azerbaijan, P.O. Box 365, Uremia, Iran
  • F. de Mattia Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, I-20126 Milan, Italy
  • M. Labra Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, I-20126 Milan, Italy

Abstract

To assess the genetic differences between clones of grapevine (Vitis vinifera L.) cv. Keshmeshi, ten selected clones
from a clonal selection programme were analysed by 23 Simple Sequence Repeats (SSR) and seven AFLP primer
combinations. No intra-varietal differences between the clones could be detected by SSRs, whereas eight out of
the 499 AFLP fragments generated by the seven primer combinations were polymorphic. The number of markers
ranged from 44 (E34-M34) to 97 (E31-M32), with an average of 71.3 fragments per primer combination. Cluster
analysis based on the AFLP data separated all the clones of Keshmeshi in two groups. The first group included nine
white berry skin clones without any genetic differences, and the second group with only a red berry skin clone.
AFLP could only distinguish the red berry clone of Keshmeshi from other white berry clones.

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Published
2016-12-12
Section
Articles