Effects of Ultraviolet C Irradiation on Stilbene Biosynthesis in Vitis vinifera L. cv. Cabernet Sauvignon Berry Skins and Calli

  • A. Xu College of Food Science and Nutritional Engineering, Beijing Key Laboratory of Viticulture and Oenology, China Agricultural University, Beijing, 100083, P.R. China
  • J.-C. Zhan College of Food Science and Nutritional Engineering, Beijing Key Laboratory of Viticulture and Oenology, China Agricultural University, Beijing, 100083, P.R. China
  • W.-D. Huang College of Food Science and Nutritional Engineering, Beijing Key Laboratory of Viticulture and Oenology, China Agricultural University, Beijing, 100083, P.R. China

Abstract

Vitis vinifera L. cv. Cabernet Sauvignon berries and calli were irradiated with ultraviolet C (UV-C) to investigate the effects on the biosynthesis of stilbene. The stilbene content in the berry skins was enhanced significantly after 10 min of UV-C irradiation over the 24 h time course, and the results varied depending on the development stage of the fruit. The maximum production of total stilbene in the berry skins occurred at 12 h after treatment, with a content of 848.45 ± 23.53 μg/g fresh weight (FW) at the beginning of véraison, 591.77 ± 26.90 μg/g FW at the end of véraison, and 170.71 ± 6.85 μg/g FW at the ripening stage. Different UV-C dosages, from 5 to 30 min, induced stilbene accumulation considerably in the calli over the 120 h experimental period, and 20 min was the most efficient. The maximum of total stilbene accumulation in the calli was 125.07 ± 3.01 μg/g FW at 96 h after 20 min irradiation. Total phenolics and total flavonoid content increased after UV-C irradiation in both the berry skins and calli. The relative expression of genes encoding enzymes involved in the branching point of stilbene and flavonoid biosynthesis was up-regulated by UV-C irradiation. The results show that UV-C irradiation significantly promotes stilbene and flavonoid biosynthesis in grape berry skins and calli, and the induction effects depend on fruit development stage and UV-C dosage.

Downloads

Download data is not yet available.
Published
2016-09-12
Section
Articles