Antibiotic Sensitivity of Grapevine: A Comparison Between the Effect of Hygromycin and Kanamycin on Shoot Development of Transgenic 110 Richter Rootstock (Vi tis B erlandieri x Vi tis rupestris)
Abstract
The effects of kanamycin and hygromycin on the in vitro development of 110 Richter rootstock (Vitis Berlandieri x V. rupestris) and derived transgenic clones were studied in order to evaluate their suitability as selectable markers for grapevine transformation. Transgenic clones carried both the neomycin phosphotransferase gene (nptll) under the nopaline synthase promoter (pNOS) and the hygromycin phosphotransferase (hpt) gene under the cauliflower mosaic virus 35S promoter (p35S), which confer the ability to grow in the presence of kanamycin and hygromycin, respectively. Even incorporated at low concentrations, both antibiotics strongly inhibited explant development for the control clone, but hygromycin appeared much more toxic. Whereas growth was stopped with 1 μg/mL hygromycin, 16 μg/mL kanamycin was required to block shoot development. Contrary to the control plants, transgenic clones exhibited a greater tolerance to hygromycin than kanamycin. In some transgenic clones the level of resistance conferred by the nptll was not significantly advantageous in terms of their ability to grow in the presence of kanamycin. Conversely, the hpt gene provided a strong level of tolerance to hygromycin in all transgenic clones, some clones developing up to 64 μg/mL hygromycin. Strategies for selection of grapevine putative transformants with these two antibiotics are discussed.Downloads
Copyright (c) 2017 South African Society for Enology and Viticulture
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
A copyright form will be e-mailed to the corresponding author when the manuscript has been accepted for publication.
In principle, the Author agrees to the following when he/she signes the copyright agreement:
I hereby assign to the SOUTH AFRICAN SOCIETY FOR ENOLOGY AND VITICULTURE (SASEV) the copyright of the text, tables, figures, supplementary material, illustrations and other information (the Material) submitted with the manuscript to be published in SOUTH AFRICAN JOURNAL OF ENOLOGY AND VITICULTURE (SAJEV) (the "Article"). The copyright becomes effective from the date the Article has been accepted for publication in SAJEV.
This is an open access journal, and the authors and journal should be properly acknowledged, when works are cited.
Author's may use the publishers version for teaching purposes, in books, theses, dissertations, conferences and conference papers.
A copy of the authors' publishers version may also be hosted on the following websites:
- Non-commercial personal webpage or blog.
- Institutional webpage.
- Authors Institutional Repository.
The following notice should accompany such a posting on the website: This is an electronic version of an article published in SAJEV, Volume XXX, number XXX, pages XXX - XXX, DOI. Authors should also supply a hyperlink to the original paper or indicate where the original paper (www.journals.ac.za/index.php/sajev/) may be found.
Authors publishers version, affiliated with the Stellenbosch University will be automatically deposited in the University's Institutional Repository SUNScholar.
Articles as a whole, may not be re-published with another journal.
The following license applies:
Attribution CC BY-NC-ND 4.0
