Selection of a New Highly Resistant Strain for Malolactic Fermentation under Difficult Conditions
AbstractMalolactic fermentation (MLF) is a biological process that contributes to wine quality, but it is frequently affected
by various vinification conditions. Resistance to four wine-limiting factors was studied with respect to 10 Oenococcus
oeni strains in order to select a suitable strain for performing reliable MLF in difficult wines. Resistance to low
fermentation temperature, high SO2 and/or ethanol concentration, and low pH were assayed in laboratory tests. A
pool of the most resistant strains was used in a set of laboratory MLFs. At the end of fermentation, the dominant
strains were identified by RAPD-PCR. The PN4 strain was found to be dominant in the majority of cases and under
the most detrimental wine conditions, and it was therefore chosen as the single-strain inoculum for the subsequent
MLF trials. The effectiveness of the PN4 strain was confirmed in a series of MLFs carried out in three different
countries under experimental and industrial conditions. It accomplished MLF in wines with up to 15.8% ethanol,
pH as low as 3.0, 60 mg/L of free SO2, and in fermentation temperatures below 17ºC. Our findings indicate that the
O. oeni PN4 strain could be an effective starter, guaranteeing regular and reliable MLF fermentation.
A copyright form will be e-mailed to the corresponding author when the manuscript has been accepted for publication.
In principle, the Author agrees to the following when he/she signes the copyright agreement:
I hereby assign to the SOUTH AFRICAN SOCIETY FOR ENOLOGY AND VITICULTURE (SASEV) the copyright of the text, tables, figures, supplementary material, illustrations and other information (the Material) submitted with the manuscript to be published in SOUTH AFRICAN JOURNAL OF ENOLOGY AND VITICULTURE (SAJEV) (the "Article"). The copyright becomes effective from the date the Article has been accepted for publication in SAJEV.
This is an open access journal, and the authors and journal should be properly acknowledged, when works are cited.
Author's may use the publishers version for teaching purposes, in books, theses, dissertations, conferences and conference papers.
A copy of the authors' publishers version may also be hosted on the following websites:
- Non-commercial personal webpage or blog.
- Institutional webpage.
- Authors Institutional Repository.
The following notice should accompany such a posting on the website: This is an electronic version of an article published in SAJEV, Volume XXX, number XXX, pages XXX - XXX, DOI. Authors should also supply a hyperlink to the original paper or indicate where the original paper (www.journals.ac.za/index.php/sajev/) may be found.
Authors publishers version, affiliated with the Stellenbosch University will be automatically deposited in the University's Institutional Repository SUNScholar.
Articles as a whole, may not be re-published with another journal.
The following license applies:
Attribution CC BY-NC-ND 4.0