Influence of Must Supplementation on Growth of Pediococcus spp. after Alcoholic Fermentation

  • M.E. Wade School of Food Science, Washington State University, Pullman, WA,
  • J.P. Osborne Department of Food Science and Technology, Oregon State University, Corvallis, OR,
  • C.G. Edwards School of Food Science, Washington State University, Pullman, Washington State University

Abstract


One factor potentially affecting growth of wine spoilage microbes (e.g., Pediococcus spp.) is the presence of nutrients not consumed during alcoholic fermentation by Saccharomyces cerevisiae. To assess the impact
of must nutrient supplementation on Pediococcus spp., synthetic grape musts containing low (55.2 mg N/L), medium (250 mg N/L), or high (530 mg N/L) concentrations of yeast assimilable nitrogen (YAN) were fermented by S. cerevisiae. Upon cessation of fermentative activity P. damnosus OW-2, P. inopinatus OW-8, P. parvulus WS-7C, WS-29A, OW-1, or P. pentosaceus ATCC 33316 were inoculated at 104 to 105 cfu/mL. With the exceptions of OW-1 and OW-2, none of the other species or strains grew in the synthetic wines unless yeast extract or peptone was added, suggesting the absence of an essential nutrient.  Experiments were replicated using Cabernet Sauvignon musts containing low (66.9 mg N/L), medium
(219 mg N/L), and high (438 mg N/L) YAN. In general, wines containing the greatest residual amino acid concentrations (high YAN) supported better growth of the aforementioned Pediococcus spp. However, low YAN wines containing negligible residual nitrogen achieved similar populations after a short period of initial inhibition, suggesting that ‘excessive’ nitrogen supplementation to musts does not have a large impact on growth of pediococci post alcoholic fermentation.

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Author Biography

C.G. Edwards, School of Food Science, Washington State University, Pullman, Washington State University

School of Food Science

Professor

References

Agenbach, W., 1977. A study of must nitrogen content in relation to incomplete fermentations, yeast production and fermentation activity. In: Proceedings of the South African Society of Enology and Viticulture, Cape Town. Stellenbosch, South Africa. pp. 66-88. South African Society of Enology and Viticulture.

Ángeles Pozo-Bayón, M., Andújar-Ortiz, I., & Moreno-Arribas, M.V., 2009. Scientific evidences beyond the application of inactive dry yeast preparations in winemaking. Food Res. Int. 42, 754-761.

Bell, S.J., & Henschke, P.A., 2005. Implications of nitrogen nutrition for grapes, fermentation and wine. Aust. J. Grape. Wine Res. 11, 242-295.

Beltran, G., Novo, M., Rozès, N., Mas, A., & Guillamón, J.M., 2004. Nitrogen catabolite repression in Saccharomyces cerevisiae during wine fermentations. FEMS Yeast Res. 4, 625-632.

Bisson, L.F., & Butzke, C.E., 2000. Diagnosis and rectification of stuck and sluggish fermentations. Am. J. Enol. Vitic. 51, 168-177.

Buechsenstein, J.W., & Ough, C.S., 1978. SO2 determination by aeration-oxidation: a comparison with Ripper. Am. J. Enol. Vitic. 29, 161-164.

Capucho, I. & San Ramao, M.V., 1994. Effect of ethanol and fatty acids on malolactic activity of Leuconostoc oenos. Appl. Microbiol. Biotechnol. 42, 391-395.

Carrete, R., Vidal, M., Bordons, A. & Constanti, M., 2002. Inhibitory effect of sulfur dioxide and other stress compounds in wine on the ATPase activity of Oenococcus oeni. FEMS Microbiol. Lett. 211, 155-159.

Charpentier, C., & Feuillat, M., 1993. Yeast autolysis. In: Fleet GH (ed.). Wine Microbiology and Biotechnology. Harwood Academic Publishers, Chur, Switzerland. pp. 225-242.

Childs, B.C., Bohlscheid, J.C., & Edwards, C.G., 2015. Impact of available nitrogen and sugar concentration in musts on alcoholic fermentation and subsequent wine spoilage by Brettanomyces bruxellensis. Food Microbiol. 46, 604-609.

Comitini, F., Ferretti, R., Clementi, F., Mannazzu, I. & Ciani, M., 2005. Interactions between Saccharomyces cerevisiae and malolactic bacteria: preliminary characterization of a yeast proteinaceous compound(s) active against Oenococcus oeni. J. Appl. Microbiol. 99, 105-111.

Conterno, L., Joseph, C.M.L., Arvik, T.J., Henick-Kling, T. & Bisson, L.F. 2006. Genetic and physiological characterization of Brettanomyces bruxellensis strains isolated from wines. Am. J. Enol. Vitic, 57, 139-147.

Davis, C.R., Wibowo, D., Fleet, G.H., & Lee, T.H., 1988. Properties of wine lactic acid bacteria: their potential enological significance. Am. J. Enol. Vitic. 39, 137-142.

Davis, C.R., Wibowo, D.J., Lee, T.H., & Fleet, G.H., 1986. Growth and metabolism of lactic acid bacteria during fermentation and conservation of some Australian wines. Food Aust. 38(1), 35-40.

De Man, J.C., Rogosa, M., & Sharpe, M.E., 1960. A medium for the cultivation of lactobacilli. J. Appl. Bacteriol. 23, 130-135.

Dukes, B.C., & Butzke, C.E., 1998. Rapid determination of primary amino acids in grape juice using an o-phthaldialdehyde/N-acetyl-L-cysteine spectrophotometric assay. Am. J. Enol. Vitic. 49, 125-134.

Edwards, C.G. & Watson, B.A., 2013. Basic Microbiological and Chemical Analyses for Wine. 82 pp. Washington State University, Cooperative Extension EM047, Pullman, WA.

Edwards, C.G., & Beelman, R.B., 1987. Inhibition of the malolactic bacterium, Leuconostoc oenos (PSU-1), by decanoic acid and subsequent removal of the inhibition by yeast ghosts. Am. J. Enol. Vitic. 38, 239-242.

Edwards, C.G., & Jensen, K.A., 1992. Occurrence and characterization of lactic acid bacteria from Washington State wines: Pediococcus spp. Am. J. Enol. Vitic. 43, 233-238.

Fugelsang, K.C., & Edwards, C.G., 2007 (2nd ed.). Wine Microbiology: Practical Applications and Procedures. Springer Science and Business Media, New York, NY.

Garvie, E.I., & Gregory, M.E., 1961. Folinic acid requirement of strains of the genus Pediococcus. Nature 190, 563-564.

Grant, C.L., & Pramer, D., 1962. Minor element composition of yeast extract. J. Bacteriol. 84, 869-870.

Hall, G., & Reuter, W.M., 2007. HPLC analysis for the monitoring of fermentation broth during ethanol production as a biofuel. PerkinElmer, Inc., Waltham, MA.

Holzapfel, W.H., Franz, C.M.A.P., Ludwig, W., & Dicks, L.M.T., 2009. Genus III. Pediococcus Claussen 1903, 68. In: Vos, P.D., Garrity, G.M., Jones, D., Krieg, N.R., Ludwig, W., Rainey, F.A., Schleifer, K-H. & Whitman, W.B. (eds.). Bergey's Manual of Systematic Bacteriology. Springer Science and Business Media, New York, NY. pp. 513-532.

Ingledew, W.M., & Kunkee, R.E., 1985. Factors influencing sluggish fermentations of grape juice. Am. J. Enol. Vitic. 36, 65-76.

Liu, S-Q., Davis, C.R., & Brooks, J.D., 1995. Growth and metabolism of selected lactic acid bacteria in synthetic wine. Am. J. Enol. Vitic. 46, 166-174.

Lonvaud-Funel, A., 1999. Lactic acid bacteria in the quality improvement and depreciation of wine. Antonie van Leeuwenhoek 76, 317-331.

Maret, R., & Sozzi, T. 1977. Flore malolactique de moûts et de vins du Canton du Valais (Suisse). I. Lactobacilles et pédiocoques. Ann. Technol. Agric. 27, 255-273.

Martínez-Rodriguez, A.J., Carrascosa, A.V., & Polo, M.C., 2001. Release of nitrogen compounds to the extracellular medium by three strains of Saccharomyces cerevisiae during induced autolysis in a model wine system. Int. J. Food Microbiol. 68, 155-160.

McWilliam, D.J., & Ough, C.S., 1974. Measurement of ammonia in musts and wines using a selective electrode. Am. J. Enol. Vitic. 25, 67-72.

Mira de Orduña, R., 2010. Climate change associated effects on grape and wine quality and production. Food. Res. Int. 43, 1844-1855.

Munoz, E., & Ingledew, W.M., 1990. Yeast hulls in wine fermentations - a review. J. Wine. Res. 1, 197-209.

Nakagawa, A., & Kitahara, K., 1959. Taxonomic studies on the genus Pediococcus. J. Gen. Appl. Microbiol. 5, 95-126.

Nel, H.A., Bauer, R., Vandamme, E.J., & Dicks, L.M.T., 2001. Growth optimization of Pediococcus damnosus NCFB 1832 and the influence of pH and nutrients on the production of pediocin PD-1. J. Appl. Microbiol. 91, 1131-1138.

Osborne, J.P., & Edwards, C.G., 2006. Inhibition of malolactic fermentation by Saccharomyces during alcoholic fermentation under low‐ and high‐nitrogen conditions: a study in synthetic media. Aust. J. Grape Wine Res. 12, 69-78.

Osborne, J.P., & Edwards, C.G., 2007. Inhibition of malolactic fermentation by a peptide produced by Saccharomyces cerevisiae during alcoholic fermentation. Int. J. Food Microbiol. 118, 27-34.

Silver, J., & Leighton, T., 1981. Control of malolactic fermentation in wine. 2. Isolation and characterization of a new malolactic organism. Am. J. Enol. Vitic. 32, 64-72.

Snell, E.E., Tatum, E.L., & Peterson, W.H., 1937a. Growth factors for bacteria: III. Some nutritive requirements of Lactobacillus delbrückii. J. Bacteriol. 33, 207-225.

Snell, E.E., Strong, F.M., & Peterson, W.H., 1937b. Growth factors for bacteria: Fractionation and properties of an accessory factor for lactic acid bacteria. Biochem. 31, 1789-1799.

Spayd, S.E., Nagel, C.W., & Edwards, C.G., 1995. Yeast growth in Riesling juice as affected by vineyard nitrogen fertilization. Am. J. Enol. Vitic. 46, 49-55.

Strickland, M.T., Schopp, L.M., Edwards, C.G., & Osborne, J.P., 2016. Impact of Pediococcus spp. on Pinot noir wine quality and growth of Brettanomyces. Am. J. Enol. Vitic. 67, 188-198.

Sturgeon, J.Q., Bohlscheid, J.C., & Edwards, C.G., 2013. The effect of nitrogen source on yeast metabolism and H2S formation. J. Wine Res. 24, 182-194.

Vilanova, M., Ugliano, M., Varela, C., Siebert, T., Pretorius, I.S., & Henschke, P.A., 2007. Assimilable nitrogen utilisation and production of volatile and non-volatile compounds in chemically defined medium by Saccharomyces cerevisiae wine yeasts. Appl. Microbiol. Biotechnol. 77, 145-157.

Von Cosmos, N.H. & Edwards, C.G., 2016. Use of nutritional requirements for Brettanomyces bruxellensis to limit infections in wine. Ferment. 2, 17.

Wade, M.E., Osborne, J.P., Strickland, M.T., & Edwards C.G., 2018. Role of Pediococcus spp. in winemaking. Aust. J. Grape Wine Res. (in press).

Wang, X.D., Bohlscheid, J.C., & Edwards, C.G., 2003. Fermentative activity and production of volatile compounds by Saccharomyces grown in synthetic grape juice media deficient in assimilable nitrogen and/or pantothenic acid. J. Appl. Microbiol. 94, 349-359.

Wibowo, D., Eschenbruch, R., Davis, C.R., Fleet, G.H., & Lee, T.H., 1985. Occurrence and growth of lactic acid bacteria in wine: a review. Am. J. Enol. Vitic. 36, 302-313.

Wibowo, D., Fleet, G.H., Lee, T.H., & Eschenbruch, R.E., 1988. Factors affecting the induction of malolactic fermentation in red wines with Leuconostoc oenos. J. Appl. Microbiol. 64, 421-428.

Published
2018-12-12
Section
Articles